Abstract

BERNAL MORENO, Sandra Carolina et al. Embryogenesis in Passiflora maliformis. Rev. peru biol. [online]. 2018, vol.25, n.3, pp.281-290. ISSN 1727-9933.  http://dx.doi.org/10.15381/rpb.v25i3.15211.

The economic, ecological and medical significance of passion fruits has encouraged the development of multidisciplinary research in this group. In vitro culture, non-zygotic embryogenesis represents an alternative for plant regeneration; however, this technique has presented difficulties in the reproducibility of protocols as well as the development of certain embryos and abnormal plantlets in some species. We assessed in Passiflora maliformis L the morphogenic capacity in the mature zygotic embryos (MZE) to develop nonzygotic embryos (NZE), and subsequent plantlets regeneration. We tested an induction stage with 12 treatments in MS + VitB5 medium supplemented with 2,4-D, KIN and BA added alone or combined and, another with a MS, MS with Activated Charcoal (AC) or MS with growth regulators (GR) in 1/10 concentrations reduced to 1/10 of those added in the induction stage. We obtained high percentages, 70%, of regenerants in the medium supplemented with 1 mgL^-1 BA and transferred to the MS expression medium, as well as in the medium with 1 mgL^-1 BA + 3 mgL^-1 2,4-D, and transferred to the expression medium with AC, 60%. 70% of the regenerated seedlings were successfully grown in the greenhouse. This is the first research that addresses the expression of the MZE embryogenic potential in P. maliformis, inducing a successfully embryogenic non-zygotic responses through direct and indirect routes; the obtained results are relevant to the knowledge of non-zygotic embryogenesis in other passion fruits, for breeding and commercial uses.

Keywords : Somatic embryogenesis; In vitro; morphogenesis; somatic regenerants; passion fruit.

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