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Revista Peruana de Medicina Experimental y Salud Publica
versión impresa ISSN 1726-4634versión On-line ISSN 1726-4642
Resumen
YANIRO, Verónica et al. Neutralization of SARS-CoV-2 (lineage B.1.1) by hyperimmune llama (Lama glama) serum in vero cell culture. Rev. perú. med. exp. salud publica [online]. 2023, vol.40, n.3, pp.287-296. Epub 27-Sep-2023. ISSN 1726-4634. http://dx.doi.org/10.17843/rpmesp.2023.403.12509.
Objective.
To evaluate the serological antibody response of a llama (Lama glama) to SARS-CoV-2 (B.1.1 lineage) immunization and the neutralizing capacity of hyperimmune llama serum against SARS-CoV-2 virus (B.1.1 lineage) in Vero cells.
Materials and methods.
A llama was immunized with inactivated SARS-CoV-2 (B.1.1 lineage). Serum samples were analyzed to evaluate the level of antibodies by ELISA, as well as reactivity to SARS-CoV-2 antigens by Western Blot. In addition, viral neutralization in cell cultures was assessed by the Plate Reduction Neutralization Test (PRNT).
Results
. Seroreactivity increased in the immunized llama from week 4 onwards. Antibody titers were the highest after the seventh immunization booster. Western blot results confirmed the positive ELISA findings, and immune serum antibodies recognized several viral proteins. The neutralization assay (PRNT) showed visible viral neutralization, which was in accordance with the ELISA and Western Blot results.
Conclusions.
The findings suggest that hyperimmune llama serum could constitute a source of therapeutic antibodies against SARS-CoV-2 infections (lineage B.1.1), and should be studied in further research.
Palabras clave : Coronavirus; SARS-CoV-2; Lama glama; Neutralization Tests; COVID-19 Serotherapy.