Resumo

MESIAS V, Fernando; SERRANO-MARTINEZ, Enrique  e  LLANCO A, Luis. Genotyping by RAPD-PCR of Yersinia ruckeri isolates from rainbow trout (Oncorhynchus mykiss) of the highlands of Peru. Rev. investig. vet. Perú [online]. 2019, vol.30, n.4, pp.1743-1749. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v30i4.17148.

The aim of this study was to identify Y. ruckeri genotypes, according to their origin, by RAPD-PCR. Aseptic samples of spleen and kidney were taken from rainbow trout (Oncorhynchus mykiss) with clinical signs of yersiniosis in fish farms of four departments (Junín, Lima, Ancash and Puno) of Peru. The strains were cultivated on trypticase soy agar at 25 °C for 24 hours and the presumptive identification of the colonies suspected of being Y. ruckeri was made by Gram stain and biochemical tests of oxidase and catalase. Then, the identity of Y. ruckeri was confirmed by PCR. In total, 63 isolates of Y. ruckeri were obtained and analyzed by RAPD-PCR. The dendogram of genetic diversity grouped all the isolates in one cluster, closely related to the control strain Y. ruckeri ATCC 29473 (96% similarity). It was also observed that all the isolates were genetically identical (clones). It is suggested that the strains could have a common origin because they had different geographical origins and high similarity between them.

Palavras-chave : fish health; RAPD-PCR; yersiniosis; isolation; Yersinia ruckeri.