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Revista de Investigaciones Veterinarias del Perú
versão impressa ISSN 1609-9117
Resumo
MONTERROSO, Michelle; SALVATIERRA R, Guillermo; SEDANO S, André e CALLE E, Sonia. Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms. Rev. investig. vet. Perú [online]. 2019, vol.30, n.1, pp.455-464. ISSN 1609-9117. http://dx.doi.org/10.15381/rivep.v30i1.15670.
The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) wereAmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated toaminoglycosideresistance mechanismwere positivein 39%(14/36) toAAC(6’),28% (10/36) toANT (2") and 11% (4/36) toAAC (3) IV.
Palavras-chave : Escherichia coli; pigs; resistance mechanisms; antimicrobial resistance; public health.