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vol.5 número1-2Aclimatación de Uncaria tormentosa (Willd.) DC. producida in vitroDiversidad genética molecular de Mirabilis expansa mediante RAPD: Molecular genetic diversity of Mirabilis expansa using RAP índice de autoresíndice de assuntospesquisa de artigos
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Ecología Aplicada

versão impressa ISSN 1726-2216

Resumo

MANSILLA S., Roberto C. et al. Molecular variabiliy analysis of a peruvian smallanthus sonchifolius (Poepp & Endl) H. Robinson "Yacon" Collection. Ecol. apl. [online]. 2006, vol.5, n.1-2, pp.75-80. ISSN 1726-2216.

Yacon is an Andean origin crop, characterized by the storage of mainly fructooligosaccharides in its roots. In spite of advancements in yacon morphological characterizations, the genetic diversity of the crop in molecular terms is still unknown. In the present study 30 cultivated accessions from the northern, center and southern regions of Peru were characterized by RAPDs. With 34 decameric primers 166 bands were generated, of which 30.7% were polymorphic. An index of similarity of 0.58 was chosen and 7 well differentiated subgroups or clusters were formed. The first cluster groups 22 accessions from the northern and southern regions with some from the center. The remaining clusters contain exclusively center region accessions. Duplicated individuals were not found and the greatest diversity was found in the center region of Peru. AMOVA reported 21.14% of variation explained by the among regions component and higher variability was concentrated among the accessions of each region (78.86%).

Palavras-chave : yacón; germplasm; molecular marker; RAPD; diversity; andean root; Smallanthus sonchifolius.

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