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Revista Medica Herediana

Print version ISSN 1018-130XOn-line version ISSN 1729-214X


ALBADO PLAUS, Emilia; SAEZ FLORES, Gloria  and  GRABIEL ATAUCUSI, Sandra. Composición química y actividad antibacteriana del aceite esencial del Origanum vulgare (orégano). Rev Med Hered [online]. 2001, vol.12, n.1, pp.16-19. ISSN 1018-130X.

Objetive: To investigate the antimicrobial activity of essential oil (Carvacrol) from Origanum vulgare. Materials and methods: The essential oil was obtained by steam water destilation dried leaves and floral; The specific gravity was obtained by a pycnometer. The refractometer Abbc was employed for the refraction Index. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS). Antimicrobial activity of O. vulgare oil was tested by semicuantittive pour plate and the agar overlay methods. Results: The specific gravity a 20°C was o,9234 and the refraction index was 1,4774. The GC-MS showed Carvacrol (9%), P-cymene (6,86%), Terpineol (12,19) and others relationated metabolic compounds. The Gram-negative bacteria: Escherichia coli, Pseudomona aeuruginosa, Salmonella tiphymurium, Salmonella cholerae suis, Vibrio cholerae and the Gram-positive Staphylococcus aureus, Bacillus cereus showed sensibility. Only Pseudomona aeruginosa showed resistance. Conclusion: The essencial oil examined had an antimicrobial activity against all bacterias evaluated, with the exception of P. aeruginosa. ( Rev Med Hered 2001; 12: 16-19 ).

Keywords : Origanum vulgare; oil essential; antibacterial activity; antimicrobial activity.

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