Fas2-ELISA y la técnica de sedimentación rápida modificada por lumbreras en el diagnóstico de la infección por Fasciola hepática

Maco Flores, Vicente; Marcos Raymundo, Luis; Terashima Iwashita, Angélica; Samalvides Cuba, Frine; Miranda Sánchez, Elba; Espinoza Babilon, Jose; Gotuzzo Herencia, Eduardo

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Revista Medica Herediana

Print version ISSN 1018-130XOn-line version ISSN 1729-214X

Abstract

MACO FLORES, Vicente et al. Fas2-ELISA y la técnica de sedimentación rápida modificada por lumbreras en el diagnóstico de la infección por Fasciola hepática. Rev Med Hered [online]. 2002, vol.13, n.2, pp.49-57. ISSN 1018-130X.

Human fasciolasis is a growing health problem, with an increasing worldwide incidence of reported cases. Improving its diagnosis through more sensitive and specific techniques is important to clinical practice and to epidemiology, specially to determine new endemic areas. Objective: To evaluate coprological techniques and serological tests for diagnosis of Fasciola hepatica (Fh) infection in humans. Material and methods: The study population involved children aged from 1 to 16 years, in a highly endemic area (Junin, Perú). A total of 194 stool samples and 158 sera were examined. Three coprological techniques were compared: formolether concentration (Ritchies), spontaneous sedimentation (SST) and Lumbreras rapid sedimentation (RST). Three serological test for Fh were evaluated: Arc 2 (double-diffsion), enzyme-linked immunoelectrotransfer blot (EITB) and Fas2-ELISA. Results: RST showed higher recovery rates (20.61%) of eggs in stools than SST (13.40%) and Ritchies (7.72%). The sensitivity of the serological tests was compared with total infected patients diagnosed by all the fecal tests: most sensitive was Fas2-ELISA with 96.77%, EITB was 71.87% and Arc 2, was 34.48%. Conclusion: We conclude that RST is better than the SST and Ritchies techniques for the diagnosis of the chronic phase of human infection by Fh using fecal examinations, and that Fas2-ELISA is a very highly sensitive immunodiagnostic test, which may be used for the diagnosis of human Fh infection in both clinical and epidemiological settings, specially for screening human populations living in endemic areas.

Keywords : Fascioliasis; Fasciola hepatica; diagnosis; serologic; coprologic; sensitivity; Lumbreras rapid sedimentation (RST); Fas2-ELISA; Perú.

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