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Revista Medica Herediana

Print version ISSN 1018-130XOn-line version ISSN 1729-214X


ALIAGA, Rocío; MIRANDA, Jacqueline  and  ZEVALLOS, Jesús. Isolation and identification of Vibrio parahaemolyticus O3: K6 in fish and bivalve mollusks of a fish market from Lima, Perú. Rev Med Hered [online]. 2010, vol.21, n.3, pp.139-145. ISSN 1018-130X.

Objective: To determine the presence of Vibrio parahaemolyticus serotype O3:K6 in fish and bivalves mollusks of a fish market from Lima. Material and methods: We analyzed 254 samples of fish and bivalve mollusks using the technique described by Yukiko Harakudo et al, consisting of an enrichment step, chromogenic plating, evaluation of glucose fermentation and salt tolerance test. The strains identified as Vibrio parahaemolyticus were serotyped using monoclonal antibodies and evaluated their pathogenic potential on Wagatsuma agar. Results: 15 strains of V. parahaemolyticus, 9 (7,5%) in fish and 6 (4,5%) in bivalve mollusks were isolated. 3 (20%) were Kanagawa phenomenon positive indicating the production of thermostable direct hemolysin (TDH). A TDH-positive strain isolated from a fish belonged to the serotype O3: K6. Conclusions: Vibrio parahaemolyticus is distributed in analyzed aquatic resources; most frequently in fish from which was found serovar O3:K6. This is the first report of pandemic O3:K6 strain of Vibrio parahaemolyticus isolated from non-human source in Perú. (Rev Med Hered 2010;21:139-145).

Keywords : Vibrio parahaemolyticus; fish; bivalves mollusks; gastroenteritis; Kanagawa phenomenon.

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