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Revista Medica Herediana

Print version ISSN 1018-130X


BAILON, Henri  and  SACSAQUISPE, Rosa. Molecular characterization of extended-spectrum beta-lactamase-producing (ESBL) strains of Klebsiella pneumoniae causing nosocomial infections in a neonatal unit in Lima, Peru. Rev Med Hered [online]. 2013, vol.24, n.2, pp.101-108. ISSN 1018-130X.

Objective: To characterize the molecular features of seven extended-spectrum betalactamase-producing (ESBL) strains of Klebsiella pneumoniae in patients with bacteremia during a nosocomial outbreak in a neonatal unit in Lima, Peru. Methods: Genotyping was performed using Eric-PCR, Rep-PCR and pulsed field gel electrophoresis (PFGE) to evaluate clonality. Disk diffusion test showed that all seven strains were ESBL producing strains. Results: The three-genotyping methods showed that five out of seven strains were genetically related to a single clone. Conclusions: The study represents the first attempt to apply genotyping methods to study nosocomial outbreaks in neonatal units in Lima, and reveals clonal transmission of ESBL producing strains of K. pneumoniae

Keywords : Klebsiella pneumoniae; cross infection; molecular typing; electrophoresis; gel; pulsed-field.

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