Abstract

OLIVA-MENACHO, José; OLIVA-CANDELA, José  and  GARCIA-HJARLES, Marco. Multi-drug resistant bacteria isolated from medical stethoscopes in a level III hospital. Rev Med Hered [online]. 2017, vol.28, n.4, pp.242-246. ISSN 1018-130X.  http://dx.doi.org/https://doi.org/10.20453/rmh.v28i4.3224.

Objectives: To determine the susceptibility profile of pathogenic bacteria isolated from medical stethoscopes in a level II hospital in Lima. Methods: Cross-sectional study carried-out in Hospital Nacional Arzobispo Loayza. One hundred and twenty-three bacteria were stored at -20C to be cultured in TSA agar and salt mannitol agar to be further incubated for 24 hours at 37C. A suspension adjusted at 0,5 McFarland turbidity was done to be cultured at Mueller - Hinton agar to determine minimal inhibitory concentration (CIM) following CLSI (Clinical and Laboratory Standard Institute) recommendations. Results: All coagulase-negative Staphylococcus spp. were susceptible to ERI, DA, CIP, NF, LZD, VAN (100%); three strains were resistant to PEN, OXA, FOX (2,8%). All five Staphylococcus aureus strains were susceptible to GE, AK, NF, LZD (100%); however, one strain was resistant to FOX, VAN (20%). The two Acinetobacter spp. strains were susceptible to IMI, MER, COL (100%). Two out of four strains of Pseudomonas were resistant to IMI, MER. Conclusions: The study reports strains of methicillin resistant coagulase negative Staphylococcus and Staphylococcus aureus; carbapenem resistant Pseudomonas aeruginosa, and cephalosporin resistant enterobacteria.

Keywords : Stethoscopes; bacterial resistance; hospital infection; Peru.

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