Abstract

OLIVA-MENACHO, José Enrique; ARROYO-ACEVEDO, Jorge Luis; OLIVA-CANDELA, José Arturo  and  GARCIA-HJARLES, Marco Antonio. Antinuclear antibody staining patterns identified by indirect immunofluorescence in patients with connective tissue disease. Rev Med Hered [online]. 2019, vol.30, n.1, pp.33-39. ISSN 1018-130X.  http://dx.doi.org/10.20453/rmh.v30i1.3470.

Objectives: To determine the antinuclear antibody staining patterns identified by indirect immunofluorescence in patients with connective tissue disease in hospital level III in Lima, Peru. Methods: Cross-sectional study performed at the Servicio de Inmunología of the Hospital Nacional Arzobispo Loayza between January and June 2017. We reviewed 291 clinical charts of patients with connective tissue disease who had determination of antinuclear antibodies by indirect immunofluorescence. Results: 322 determinations were analyzed; patterns detected were PCNA 6 (1.86%); homogeneous pattern 109(33.85%), centromeric pattern 34(10.56%), speckled pattern 135(41.93%), cytoplasmic pattern 25(7.76%), nucleolar pattern 9(2.80%), NUMA 1- Huso achromatic pattern 3(0.93%) and lysosomal pattern 1(0.31%). The spectrum of diseases in which antinuclear antibodies were looked for were systemic lupus 85(29.21%), limited progressive systemic sclerosis CREST) 34(11.68%), Sjögren´s syndrome 77(26.46%), scleroderma 15(5.15%), mixed connective tissue disorders 72 (24.74%) and polymyositis 8 (2.75%). Conclusions: A high frequency of homogeneous and speckled patterns were observed, the former associated with systemic lupus and the latter with Sjögren´s syndrome. Detection of antinuclear antibodies by indirect immunofluorescence is an accurate diagnostic method.

Keywords : Antibodies; antinuclear; fluorescent antibody technique; indirect; undifferentiated connective tissue diseases.

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