Abstract

CHURATA-OROYA, Diana Eugenia et al. Antifungal Effect of Citrus paradisi “grapefruit” on strains of Candida albicans isolated from patients with denture stomatitis. Rev. Estomatol. Herediana [online]. 2016, vol.26, n.2, pp.78-84. ISSN 1019-4355.  http://dx.doi.org/10.20453/reh.v26i2.2869.

Objectives: To determine the in vitro antifungal activity of essential oil of Citrus paradisi “grapefruit” on Candida albicans strains isolated from patients diagnosed with denture stomatitis. Material and methods: Sensitivity tests were performed by the agar diffusion method with wells. Candida albicans strain was obtained from swab samples of mucosa of patients with denture stomatitis and identified by morphological characteristics of colonies and germ tube test. The inoculum was standardized to 0.5 Mc Farland scale and seeded in 12 well plates with Sabouraud dextrose agar. wells a punch of 6 mm diameter were made, 50 uL of the different concentrations of essential oil were deposited and incubation proceeded to aerobically at 37 ° C for 48 hours. Statistical analysis was performed using SPSS version 23. Results: concentrations 25; 12.5; 6.25; 3,13 and 1,56% had a halo of inhibition average of 12.6; 10.3; 7.8 and 6.8 mm respectively, while 0.78 and 0.39% had no antifungal activity. The MIC for Candida albicans average was 6.25% .v / v Kruskal Wallis test was performed and it was determined that there is statistically significant difference p <0.05 between concentrations of averages. Conclusions: It was determined that the essential oil of Citrus paradisi “grapefruit” presents antifungal activity on strains of Candida albicans.

Keywords : Citrus paradisi; antifungal agents; Candida albicans; stomatitis denture.

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