Abstract

CORADI TONON, Caroline et al. Antifungal activity of a-terpinen on Candida albicans. Rev. Estomatol. Herediana [online]. 2019, vol.29, n.2, pp.107-114. ISSN 1019-4355.  http://dx.doi.org/https://doi.org/10.20453/reh.v29i2.3527.

Objective: to evaluate the antifungal activity of a-terpinen on planktonic cultures and biofilm of Candida albicans. Material and Methods: first, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (CFM) of a-terpinen were determined. Nystatin was used as a positive control. Biofilm of Candida albicans was developed and, after treatment with different concentrations of a-terpinen, was quantified in CFU/mL, in addition to metabolic activity of the cells being evaluated by XTT. Results: the lowest concentration able to inhibit the growth (MIC) was 0.2% for a-terpinen and 4 µg / mL for Nystatin. Results showed that from the concentration 0.05% of α -terpinen and 2 µg / mL of Nystatin, there was a decrease of Candida albicans when compared to the control, in planktonic culture. CFM was 0.2% for α -terpinen and 8 µg / mL for Nystatin. Regarding the quantification, effective concentrations were α-terpinen (0.1%) and Nystatin (128 µg/mL), and in the XTT test, α-terpinen (0.1%) and Nystatin (256 µg/mL) decreased metabolic activity when compared to control. Conclusion: Thus, it can be stated that a-terpineol may be an alternative for the treatment of fungal infections.

Keywords : Candida albicans; biofilm; nystatin.

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