Abstract

VILLANUEVA, Orlinda  and  ARNAO, Inés. Purification of a 35 kDa protein rich in lysine from Amaranthus caudatus (kiwicha) albumin fraction. An. Fac. med. [online]. 2007, vol.68, n.4, pp.344-350. ISSN 1025-5583.

Objective: To purify a protein rich in lysine from Amaranthus caudatus seeds’ albumin fraction. Design: Observational descriptive study. Setting: Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos. Biologic material: Amaranto (Amaranthus caudatus) seeds, Oscar Blanco variety. Interventions: We used both chromatographic (gel filtration) and electrophoretic (SDS-PAGE and electroelution) techniques to purify a protein rich in lysine and to determine its molecular weight. The aminoacid composition of the lysine rich protein was obtained by high pressure liquid chromatography (HPLC). Main outcome measures: Isolation and purification of a 35 kDa protein rich in lysine. Results: We isolated a 35 kDa molecular weight protein as determined by SDS-PAGE; the essential amino acid composition was similar to the standard protein recommended by the World Health Organization (WHO) with high content of lysine (8,61 moles %). Conclusions: We have isolated and purified a 35 kDa protein rich in lysine from Amaranthus caudatus albumin fraction with an essential amino acid composition similar to that recommended by WHO.

Keywords : Amaranthus; albumins; lysine; amino acids; amino acids, essential.

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