SciELO - Scientific Electronic Library Online

 
vol.69 número4Nivel de pobreza asociado al estadio de gravedad del cáncer ginecológicoHepatoprotección antioxidante de la cáscara y semilla de Vitis vinifera L. (uva) índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Bookmark


Anales de la Facultad de Medicina

versión impresa ISSN 1025-5583

Resumen

HUERTA, Doris et al. Relation of the hepatic lipase gene C-514T polymorphism with nutritional indicators and lipoproteins in a Peruvian population sample: a nutrigenetic perspective. An. Fac. med. [online]. 2008, vol.69, n.4, pp. 244-249. ISSN 1025-5583.

Introduction: Cardiovascular diseases are major causes de death in the world. The hepatic lipase (HL) gene promoter region presents a C-514T functional polymorphism related to enzyme activity, variation of lipoproteins levels and possible cardiovascular disease risk. Objectives: To determine the association of HL gene promoter region polymorphism with both nutritional indicators and lipoproteins levels in a healthy Peruvian sample. Design: Descriptive, transversal, associative study. Setting: Alberto Guzman Barron Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos. Participants: Ninety healthy male and female volunteers aged 18 to 58 years. Interventions: Genomic DNA was obtained from serum samples according to standard methodology. Anthropometric measurements and lipid profile by enzymatic methods were performed. Polymorphism C-514T in the HL gene was determined by polymerase chain reaction (PCR). PCR products were digested with NlaIII and fragments separated by polyacrilamyde gel electrophoresis (PAGE) and stained with silver nitrate. Main outcome measures: HL gene genotypes and alleles frequencies and relation with both lipid and nutritional parameters. Results: We found genotype frequencies CC=0,143; CT=0,593 and TT = 0,264, consistent with Hardy-Weinberg equilibrium (X2 =3,8024, g.l. = 1, p = 0,086). Alleles frequencies were C allele = 0,4395 and T allele = 0,5605. HDLc, LDLc, TAG cholesterol levels and subcutaneous fold, BMI and fat percentage averages in CC, CT and TT genotypes did not show significant differences (p>0,05). Nevertheless, when T allele was analyzed alone (genotypes CT and TT) according to age and sex there were significant differences (p<0,05) in some parameters. Conclusions: CT heterozygote and T allele, related to HL low activity were the most frequent genotypes in this Peruvian sample; in addition, presence of T allele was associated with variations in lipoprotein levels and nutritional factors which could have influence on cardiovascular diseases.

Palabras llave : Genetic; hepatic lipase; polymorphism, genetic; lipoproteins; cardiovascular diseases.

        · resumen en Español     · texto en Español     · pdf en Español