Resumo

HUAMAN, Oscar et al. Effect of Bixa orellana (achiote) leaves aquous and hydroetanolic extracts on non-enzymatic indicators of paracetamol hepatoxicity in rats. An. Fac. med. [online]. 2013, vol.74, n.4, pp.279-283. ISSN 1025-5583.

Objectives: To determine the effect of Bixa orellana leaves aqueous and hydroethanolic extracts on non-enzymatic indicators of hepatotoxicity in rats subjected to acetaminophen. Design: Experimental. Setting: Research Center of Biochemistry and Nutrition, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biologic material: Thirty-five male rats aged 3 months. Interventions: Rats were randomized into 5 groups that received orally and for 10 days: positive and negative controls NaCl 0.9%, silymarin 300 mg/kg, aqueous extract 500 mg/kg and hydroethanolic extract 500 mg/kg; after fasting 24 hours, paracetamol 400 mg/kg peroral was administered at the fifth day, except for the negative control. Under ether anesthesia cardiac puncture was performed to obtain blood. Main outcome measures: Liver ratio (liver weight/animal weight x 100), total bilirubin (TB), direct (BD) and indirect (BI) bilirubin, hepatomegaly, thiobarbituric acid reactive species (TBARS) in liver and serum. Results: Treatment with aqueous extract alone decreased the following indicators: BT and BI (p<0.01), serum TBARS (p<0.05), hepatomegaly -13.2% (p<0.01). The group receiving hydroethanolic extract reduced BT, BI (p<0.01), BD (p<0.05), TBARS in liver (p<0.01), hepatomegaly -9.37%. Conclusions: Bixa orellana (achiote) leaves aqueous and hydroethanolic extracts showed hepatoprotective effect against paracetamol in toxic doses in rats.

Palavras-chave : Bixa orellana; TBARS; bilirubin; liver index; hepatoprotective.

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