Resumen

PACHECO-ROMERO, José et al. Association between vascular endothelial growth factor (VEGF) gene polymorphism and preeclampsia. An. Fac. med. [online]. 2014, vol.75, n.2, pp.119-123. ISSN 1025-5583.

Background: Preeclampsia (PE) is the first cause of maternal death in the world and affects about 10% of pregnant women in some Peruvian regions. Various genes are involved en PE risk, including the vascular endothelial gene factor (VEGF). Objetives: To study VEGF gene +936 CT polymorphism and to determine its association with preeclampsia. Design: Observational, relational (associative), casecontrol (non-experimental) study. Setting: Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Pregnant women with and without preeclampsia. Methods: The sample included 94 pregnant women (45 with PE and 49 controls without PE) attended at Hospital Docente Madre-Niño San Bartolome. Following informed consent, 3-5 mL of blood was collected. DNA was isolated by standard methods. VEGF polymorphism was determined by PCR-RFLP and electrophoresis in either agarose or polyacrylamide gels. Main outcome measures: Association between VEGF genotypes and alleles and preeclampsia. Results: CC, CT y TT genotypes distribution in the Cases group were in ‘Hardy-Weinberg imbalance’ (there was a factor influencing that distribution), while genotypes in the Control group were in equilibrium. Cases and controls VEGF genotypes frequencies showed non-significant differences within significance limits (X2=5.630, p=0.060). TT homocygote was the most frequent genotype present in cases and CT heterocygote genotypes the most frequents in controls. C and T alleles frequency differences in cases and controls were not significant (X2=0.614, p=0.434). Conclusions: There was no association between VEGF genotypes (but in significance limit) and alleles and preeclampsia in the sample studied. Results have to be confirmed and studies in other Peruvian subjects are necessary.

Palabras clave : Preeclampsia; VEGF; polymorphism; gene.

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