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Anales de la Facultad de Medicina

Print version ISSN 1025-5583


ROJAS ARMAS, Juan et al. Thymus vulgaris L (thyme) essential oil and its combination with EDTA against Gandida albicans; formulation of a cream. An. Fac. med. [online]. 2015, vol.76, n.3, pp.235-240. ISSN 1025-5583.

Introduction: Candida albicans may cause severe invasive inlections and Irequently superficiallesions. Drugs used in treatment present drawbacks and search of alternatives becomes necessary. Objectives: To determine the activity of Thymus vulgaris essential oil (EO) alone and combined with EDTA on Candida albicans, and lormulate a cream with EO supplemented with natural terpenes. Design: In vitro experimental and in vivo toxicity study. Setling: Faculty of Medicine and Faculty 01 Pharmacy, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Candida albicans strain for in vitro study and rabbits for dermal toxicity tests. Methods: Antifungal activity was determined by agar well diffusion method, and minimum inhibitory concentration (MIC) by broth microdilution method. A cream was formulated with EO 1% and another supplemented with carvacrol, thymol, citral and EDTA. Dermal toxicity in rabbits was determined. Main outcome measures: Inhibition halos, MIC, signs 01 toxicity. Results: EO at 100 mg/mL inhibited Candida albicans growth with 30.33 ± 0.58 mm inhibition halos; adding EDTA produced 35.33 ± 0.58 mm halos. MIC for EO and EO + EDTA was respectively 8 mg/mL and 0.5 mg/mL. EO 1 % cream originated 13.33 ± 0.58 mm inhibition halos, and addition of EDTA, thymol, carvacrol and citral increased halos to 24.33 ± 0.58 mm. No signs of dermal toxicity in rabbits were observed. Conclusions: Thymus vulgaris EO showed inhibitory activity against Candida albicans and EDTA increased this activity; essential oil, EDTA, carvacrol, thymol and citral lormulated in cream al so showed synergistic effect without evidence of dermal toxicity.

Keywords : Thymus; Thyme; Candida albicans; EDTA; Cream; Rabbits.

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