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Anales de la Facultad de Medicina
Print version ISSN 1025-5583
Abstract
MARTINEZ-ROJAS, Rosa; MONDRAGON-MARTINEZ, Aarón; PULIDO-MURILLO, Eduardo A. and TANTALEAN-VIDAURRE, Manuel. Characterization of the protein pattern and antigen profile of L 3 larvae of medical importance in anisakids isolated from commercial fish of the peruvian coast. An. Fac. med. [online]. 2020, vol.81, n.3, pp.324-329. ISSN 1025-5583. http://dx.doi.org/10.15381/anales.v81i3.17256.
Introduction.
Anisakidosis is a zoonosis caused by accidental ingestion of anisakid larvae (L3).
Objective.
To characterize the protein pattern and antigenic profile of the L3 of Anisakis simplex s.l. (type I), A. physeteris s.l. (type II) and Contracaecum osculatum s.l. commercial3 fish isolated.
Methods.
An SDS-PAGE electrophoretic run of the somatic antigens was performed. Rabbits were immunized experimentally and evaluated by EITB.
Results.
The protein pattern of Anisakis type I showed 12 bands, 18 Anisakis type II and C. osculatum 13, with bands 10 and 35 kDa specific for Anisakis type II, 28 and 77 for C. osculatum, not present in Anisakis type I.
Conclusion.
Specific immunogenic bands were determined for Anisakis type I with the molecular weight proteins 11, 14, 25 and 40 kDa, for type II of 9, 10, 12, 35 and 41 kDa and C. osculatum 13, 15, 17, 30 and 47 kDa.
Keywords : Anisakis; Antigen; Immunoelectrophoresis; Western Blotting; Peru.
