Abstract

CAMARGO C, Iván; RIVERA G., Hermelinda  and  BENITO Z, Alfredo. Detección de animales portadores del virus del cólera porcino en una granja tecnificada del valle de Lima. Rev. investig. vet. Perú [online]. 2002, vol.13, n.2, pp.56-60. ISSN 1609-9117.

The objective of this study was to identify animal carriers of the Hog Cholera virus (HCV) post vaccination in a well-managed pig farm of Lima valley. A total of 166 serum samples were collected from 166 piglets between 6 and 7 weeks of age, vaccinated against Hog Cholera 15 days before sampling. Specific HCV antibodies were detected using a blocking ELISA test. Eighty eight (146/166) of animals reacted positively against HCV; 3% (5/166) and 9% (15/166) of animals were considered suspects and negatives to antibodies, respectively. A second sample was collected 30 days after the first collection from suspect (n=5) and negative (n=15) animals. HCV was detected by direct inmunofluorescence test using cultivated lymphocytes. At the time of the second sampling, 14 out of 20 animals stayed at farm. Antibodies were detected in 6 animals and 8 were negative, however 4 of the latter were positive to HCV. The results showed that the frequency of HCV carrier animals was 2.4% (4/166). The lack of antibodies and the presence of HCV in lymphocytes after vaccination, suggested that those animals were persistently infected and HCV carriers.

Keywords : Hog cholera virus; antibody; antigen; carrier; blocking ELISA test.

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