Abstract

ARIAS C., Nino  and  HUANCA L., Wilfredo. A Method for Sexing Alpaca DNA by PCR Amplification of the SRY Gene. Rev. investig. vet. Perú [online]. 2009, vol.20, n.2, pp.203-207. ISSN 1609-9117.

A method for determining the sex of DNA samples from alpacas has been developed using amplification of the SRY (Sex-determining Region Y chromosome) gene. New oligonucleotide primers were designed according to the SRY gene partial sequence from guanaco (Lama guanicoe). After electrophoresis, a normal male alpaca showed a single SRY band of 146 bp that did not appear in female alpaca samples. After optimization, the PCR procedure for determination of sex was applied to 10 DNA alpaca samples. The sex (genotypic) as diagnosed by PCR corresponded with anatomical sex (phenotypic) in all cases. This study showed that the present method can be applied for sexing DNA samples from alpacas by PCR amplification of the SRY gene.

Keywords : bioinformatics; molecular diagnosis; PCR; SRY; Y chromosome; alpaca.

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