SciELO - Scientific Electronic Library Online

 
vol.22 issue2Patterns of spermatozoa movement in thealpaca (Vicugna pacos): ex situ observationsGastric, liverand renal lesions associated to the syndrome of gastric ulcers in thoroughbred horses author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Revista de Investigaciones Veterinarias del Perú

Print version ISSN 1609-9117

Abstract

CERVANTES F., Miriam et al. Relationship between day of collection and embryo recovery in superovulated alpacas. Rev. investig. vet. Perú [online]. 2011, vol.22, n.2, pp.125-132. ISSN 1609-9117.

The relationship between day of collection and embryo recovery was studied in multiparous adult alpacas. Females with ≥7 mm follicles were superovulated, mated with fertile males (day 0 = day of service) and embryos were recovered on day 5 (G1), 6 (G2) and 7 (G3) post copula, where the number and size of corpora lutea were echographically evaluated. In Exp. 1, 14 females were slaughtered and embryos were recovered from the uterine horns and the oviduct. In Exp. 2, embryos were recovered in vivo from 21 females. Each uterine horn was flushed with 250 ml of PBS using a Foley catheter; and oviducts were flushed from the utero-tubal junction to the fimbria using 20 ml of PBS. The number and rate of embryos recovered from the uterine horns was higher in G2 and G3 as compared to G1 (p<0.05), while in the oviduct was higher in G1. Collected embryos from uterine horns had the condition of excellent and good in 81.4% (35/43) in Exp. 1 and 74.5% (38/51) in Exp. 2, and without statistical differences between treatments in each experiment. The most frequent level of embryo development was hatching blastocyst (77.7%, 73/94), and without differences between G2 and G3. It was concluded that embryos could be recovered until day 5 after mating in oviducts and from day 6 onwards in the uterine horns.

Keywords : alpaca; eCG; collection day; embryo recovery; uterine horn; oviduct.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )