Revista de Investigaciones Veterinarias del Perú
ISSN 1609-9117 versión impresa
ROMERO J, Jaime, CHAVEZ V, Amanda, CASAS A, Eva et al. RESPUESTA INMUNE EN CONEJOS A DOS TAMAÑOS DE QUISTES DE SARCOCYSTIS AUCHENIAE. Rev. investig. vet. Perú, abril./jun 2012, vol.23, no.2, p.220-227. ISSN 1609-9117.
The aim of this study was to evaluate the immune response of small and large macrocysts of Sarcocystis auchenie using three protocols of inoculation. Alpaca meat samples with S. aucheniae were obtained from the slaughterhouse of Huancavelica, Peru. Cysts were collected and classified by size in large (>5 mm) and small (1-3 mm), obtaining 3.23 and 2.45 mg/ml respectively of antigenic protein by the Biuret method. Fifteen rabbits were immunized with 0.05 mg/kg of antigenic protein derived from large and small macrocysts. Besides, three protocols of inoculation were considered (n = 5 rabitts). Protocol I and II were of 1 and 3 month length where proteins were inactivated with formalin, and protocol III of 2 months length using heat-inactivated antigen protein. Specific immune response Ag-Ab was detected by double immunodiffusion in agar gel in 100% of rabbits using protocols I and II and 60% with protocol III in large macrocysts while 100, 60 and 40% response in protocols I, II and III in small macrocysts respectively. In conclusion, immune responses of 86.6 and 66.6% were obtained when using antigenic proteins from large and small cysts, respectively; however, no association was observed between protocols and Ag-Ab reaction when using Fisher’s analysis.
Palabras llave: Sarcocystis aucheniae; macrocystic; antigenic proteins; immune response; inoculation protocols.
Universidad Nacional Mayor de San Marcos
Facultad de Medicina Veterinaria
Av. Circunvalación Cdra. 28 s/n - San Borja
Apartado 03-5137 - Lima - Perú
Telf.: (511) 435-3348 anexo 236
Fax: (511) 6197000 anexo 5017