SciELO - Scientific Electronic Library Online

 
vol.24 issue3Frequency of enterobacteria in fresh vegetables of raw consumption sold in four markets in Lima, PeruTraceability, veterinary public health and food security: an integral approach author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

Share


Revista de Investigaciones Veterinarias del Perú

Print version ISSN 1609-9117

Abstract

SHIVA, Carlos  and  JARA, Luis M. Identification and molecular characterization of native strains of lactobacillus isolates from poultry litter. Rev. investig. vet. Perú [online]. 2013, vol.24, n.3, pp.307-315. ISSN 1609-9117.

The aim of this study was to isolate and identify bacteria of Lactobacillus genus from poultry litter with 1, 2 or 3 uses and low ammonia records. Resistance for conditions at pH 5 and 8 was evaluated as well as the ability to inhibit activity in urease bacteria (Staphylococcus spp and Pseudomona spp) isolated from poultry litter. Seventy three suspected colonies of Lactobacillus were identified by culture in selective media and morphological patterns. Gram staining and catalase test revealed that only 19 strains were of Lactobacillus genus. Furthermore, through PCR was found that 53% corresponded to L. reuteri, 32% to L. plantarum, 10% to L. vaginalis and 5% to L. salivarius. All isolates survived at conditions of pH 5 and 8 and all strains produced some type of active biocin against Staphylococci spp or Pseudomona spp which might be useful as additive to regulate the microbiota in poultry litter.

Keywords : Lactobacillus; broiler; probiotics; isolation; ammonia.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )