Abstract

CANORIO PARIONA, Nadia; PAREDES ARNEDO, Fernando  and  VALDIVIA CUYA, Martha. Alternative cryoprotectans suitable for an slow freezing method of epididymal spermatozoa of alpaca (Vicugna pacos). Rev. investig. vet. Perú [online]. 2015, vol.26, n.3, pp.434-443. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v26i3.11185.

The objective of this study was to use alternative cryoprotectans suitable for freezing epididymal spermatozoa of alpaca (Vicugna pacos) in a slow freezing method. Epididymides obtained in the slaughterhouse of Huancavelica city were transported at 4 °C in saline solution to the laboratory in Lima, Peru. The sperm cells were extracted from the epididymis and incubated in HAMF10 medium. The Tes-Tris-Yolk-Citrate medium was used and supplemented with the cryoprotectants dimethyl sulfoxide (Me2 SO4 ) at 0.5, 0.25 and 0.125M concentrations, and dimethylacetamide (DMA) at 0.75, 0.385 and 0.18M concentrations, and compared with a control group cryopreserved with glycerol 0.6M. Motility, viability and integrity of membrane before and after freezing were assessed. The results showed a better viability and membrane integrity post-freezing when using DMA 0.375M (p<0.05). Motility was better with Me2 SO4 0.25 and 05M and DMA 0.375 and 0.75M (p<0.05)

Keywords : alpaca; sperm; cryopreservation; dimethylacetamide; dimethylsulfoxide.

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