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Revista de Investigaciones Veterinarias del Perú
versão impressa ISSN 1609-9117
Resumo
ALMEYDA R, Marcos; ROSADIO A, Raúl e MATURRANO H, Lenin. Genotypes of Kappa-Casein gen in creole cattle of Bambamarca district (Cajamarca, Peru). Rev. investig. vet. Perú [online]. 2016, vol.27, n.1, pp.82-90. ISSN 1609-9117. http://dx.doi.org/10.15381/rivep.v27i1.11452.
The aim of this study was to determine the allelic and genotypic frequency of kappacasein gene (κ-CN) in creole cattle. For this purpose, 48 blood samples were collected from animals in nine villages of the Bambamarca district, Cajamarca, Peru. DNA was extracted and genotyping was performed by PCR-RFLP analysis of κ-CN gene. Genotype frequencies of 0.56, 0.27 and 0.17 for the AB, AA and BB genotypes were found, while the allele frequencies were 0.552 and 0.448 for alleles A and B respectively. It was concluded that the tested group had a low proportion of individuals with the desired BB genotype that favors the production of cheese.
Palavras-chave : creole cattle; kappa casein gene; PCR-RFLP.