Abstract

USUGA S, Alexandra; RESTREPO B, Giovanni  and  A. ROJANO, Benjamín. Cryotolerance of stallion semen, oxidative stability and components of seminal plasma. Rev. investig. vet. Perú [online]. 2016, vol.27, n.3, pp.505-517. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v27i3.12005.

Contribution of seminal plasma on stallion semen cryotolerance is largely unknown. Some components of seminal plasma are known for their antioxidant effect and therefore may influence the oxidative stability of lipids and proteins of semen. The aim of this research was to evaluate the relationship between the oxidative stability and some components of seminal plasma with stallion semen cryotolerance. In seminal plasma of 30 horses were assessed vitamin C content (VC) by HPLC, total protein (TP) by Bradford, and protein and lipid oxidation (PO and LP) by TBARS and carbonyls tests, respectively. The semen was frozen with 10% of seminal plasma and post-thaw semen quality was evaluated. Adjustment of generalized linear models (GLM), regression and correlation analysis, and comparison of means by Tukey were performed. Averages of 7.13 mg BSA/ml and 6.05 mg/l for PT and VC respectively were found. Results of lipids and proteins oxidation were 3.27 nmol MDA/l and 0.03 nmol carbonyls/g protein, respectively. Association between VC, TP, LP and PO with various parameters of post-thawing semen quality were found (p<0.05). It is concluded that levels of total protein, vitamin C and seminal plasma oxidation are associated with stallion semen cryotolerance

Keywords : vitamin C; oxidative stress; freezing; spermatozoa.

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