Abstract

DUQUE C, Juan Esteban; ROJANO, Benjamín A  and  RESTREPO B, Giovanni. Cryotolerance of Stallion Semen Frozen with Addivites in the Extender. Rev. investig. vet. Perú [online]. 2017, vol.28, n.1, pp.120-129. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v28i1.12944.

Extenders have been established as an alternative to compensate the antioxidant deficiencies of stallion semen during freezing. Several research works have aimed to evaluate the effect of various additives with antioxidant properties on the equine sperm cryotolerance; however, in limited occasions this has been linked with the extender total antioxidant capacity (TAC). This research aimed to evaluate the effect of two additives on freezing extender TAC and stallion semen cryotolerance. Semen of five Colombian Creole horses was frozen in an extender under three treatments: T0: control, T1: quercetin 100 µM, T2: phosphates and carbonates (Na HPO 0.32 mg/ml + KH₂ PO₄ 0.67 mg/ml + K₂ CO₃ 0.39 mg/ml). TAC of extender was evaluated by the ABTS test. Post-thaw motility and kinetics, abnormal morphology (AM), and structural (MSI) and functional (HOS) plasma membrane integrity were assessed. The results were analysed using mixed models. TAC values (µmol trolox/l) for T0, T1 and T2 of 10890 ± 210, 14260 ± 410 and 14670 ± 410 were found. The T2 had higher values for most parameters of motility and membrane integrity (p<0.05). It is concluded that the addition of phosphates and carbonates increases the freezing extender TAC and improves stallion semen cryotolerance.

Keywords : extender; cryopreservation; carbonates; phosphates; quercetin.

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