Abstract

ANDRADE, Priscila; ELIAS P, Roberto; GRANDEZ R, Ricardo  and  MAMANI P, Javier. Histological description of the skin of the Titicaca water frog (Telmatobius culeus). Rev. investig. vet. Perú [online]. 2018, vol.29, n.1, pp.64-74. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v29i1.14085.

This study describes the morphological and histochemical characteristics of the skin of the Titicaca water frog Telmatobius culeus. Skin samples were taken from six body regions of 20 specimens. The histological techniques used were with Hematoxylin-Eosin staining (HE) to describe the morphological characteristics of the skin; Alcian Blue pH 1.0 for the detection of sulphated muco-substances and pH 2.5 for detecting acidic mucosubstances; Schiff Periodic Acid (PAS) for neutral and acid (hydroxyl) mucopolysaccharides; Toluidine Blue for the identification of sulfated, carboxylate and phosphate acids, and to observe the presence of metachromacy, which indicates the presence of anionic or polyanionic acids; and Mallory’s trichrome for the description of connective tissue. The skin samples had similarities with morphological characteristics of other amphibians described in previous studies: the dorsal and parotid regions have the greatest thickness of dermis and the two types of glands found denote being heterogeneous mucous and serous.

Keywords : Telmatobius coleus; skin; glands; histology; histochemistry.

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