Abstract

RIVERO-RODRIGUEZ, Matilde Elena; RODRIGUEZ-JIMENEZ, Jorge Luis; PEREZ-DORIA, Alveiro  and  ELIAS BEJARANO, Eduar. Isolation of Leishmania Infantum From Canis Familiaris in an Urban Area of the Colombian Caribbean. Rev. investig. vet. Perú [online]. 2018, vol.29, n.3, pp.923-930. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v29i3.13708.

The isolation of parasites of the genus Leishmania is reported from a popliteal ganglion biopsy obtained from a dog with canine visceral leishmaniasis. The dog lived in the urban area of the municipality of Ovejas, Sucre, Colombia. The Indirect Immunofluorescence test was positive for anti-Leishmania spp antibodies up to a dilution of 1/128. The typing of L. infantum was performed by isoenzyme electrophoresis and by analysis of the sequence of the cytochrome b gene and the nuclear gene that codes for the heat shock protein of 70 Kda (Hsp70). The present finding is the first confirmation of infection by L. infantum in canines in the urban area of the Colombian Caribbean.

Keywords : L. infantum; dog; leishmaniasis; IFAT; isoenzymes; PCR.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )