Abstract

CONTRERAS, Winnie; GARCIA, Luis Ruiz  and  SANTIANI, Alexei. Evaluation of dimethylacetamide and dimethylformamide as cryoprotectants for epididymal alpaca sperm (Vicugna pacos). Rev. investig. vet. Perú [online]. 2020, vol.31, n.1, e17548.  Epub Mar 31, 2020. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v31i1.17548.

The aim of this study was to determine the effect of the type (dimethylacetamide [DMA] and dimethylformamide [DMF]) and concentrations (1, 3.5, 7%) of cryoprotective agents on three sperm parameters during the cryopreservation process of epididymal alpaca sperm. A 2x3 factorial design consisting of the two cryoprotectants and the three concentrations was used. The parameters of sperm viability (SYBR-14/PI) and mitochondrial membrane potential (MitoTracker Deep Red FM) of sperm of 40 epididymis were evaluated by flow cytometry and motility by microscopy after thawing. A higher percentage of motility after thawing was found with AMD (14%) than with DMF (10%) (p=0.001). In addition, there was a significant effect of the cryoprotectant concentration in all the parameters evaluated (p<0.001), being better at concentrations of 1 and 3.5% than with 7%. No significant effect of the cryoprotectant-concentration interaction was found.

Keywords : Vicugna pacos; cryopreservation; spermatozoa; epididymis; dimethylacetamide; dimethylformamide; cytometry.

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