Abstract

CHINCHILLA, Danny; THOMAS, Richard  and  CASTRO, Lyda R.. Real time PCR for detection of Hepatozoon canis in dogs from the city of Cúcuta, Colombia. Rev. investig. vet. Perú [online]. 2020, vol.31, n.3, e16429. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v31i3.16429.

Hepatozoon canis are protozoa transmitted to dogs mainly by ticks of the Rhipicephalus sanguineus species. In Colombia, H. canis has been detected; however, only one study using the PCR technique is available detecting this hemoparasite. This study reports a frequency of infection of 8.6% (30/350 samples). H. canis was detected in blood samples from domestic dogs in the city of Cucuta (Colombia), by real-time PCR using hybridization probes with duplex detection and internal control of GADPH amplification, using primers and probes designed in this study.

Keywords : Apicomplexa; canines; molecular detection; hemoparasites.

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