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Revista de Investigaciones Veterinarias del Perú

Print version ISSN 1609-9117

Abstract

MEZA R, Dante  and  MORALES-CAUTI, Siever. Identification, serotyping and determination of the sensitivity profile of Salmonella enterica isolated from cloacae of red-eared slider turtles (Trachemys sp) in captivity, Peru. Rev. investig. vet. Perú [online]. 2020, vol.31, n.4, e19022. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v31i4.19022.

The aim of this study was to identify, serotype and determine the sensitivity profile of Salmonella enterica strains isolated from red-eared slider turtles (Trachemys sp) from two breeding centres in Lima, Peru, through cloacal swabs. The samples were isolated using tetrathionate enrichment broth, specific media such as Salmonella-Shigella agar and xylose lysine deoxycholate agar. The identification was made through biochemical tests. Serotyping through the recognition of somatic (O) and flagellar (H) antigens of the Kauffmann-White system, and the determination of sensitivity using the Kirby Bauer diffusion disk test. The results showed that 6.1% (4/66) of Salmonella enterica isolates were obtained, identifying the serotypes Salmonella Saintpaul and Salmonella Infantis. Antibacterial sensitivity tests indicated differences between strains, showing 100% sensitivity against nalidixic acid, norfloxacin and enrofloxacin and less for streptomycin, sulfamethoxazole + trimethoprim, chloramphenicol and amoxicillin + clavulanic acid. The results show the potential risk to public health involved in raising these turtles as potential pets.

Keywords : Salmonella enterica; Trachemys sp; serotyping; Infantis; Saintpaul.

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