SciELO - Scientific Electronic Library Online

 
vol.32 número2Características epidemiológicas y clínicas de las infecciones por dengue y zika durante el fenómeno de El Niño Costero de 2017 en Chincha, PerúDeterminación del pH y recuento de mesófilos en carne de boquichico (Prochilodus nigricans) proveniente de los mercados de la ciudad de Pucallpa, Ucayali índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

Compartilhar


Revista de Investigaciones Veterinarias del Perú

versão impressa ISSN 1609-9117

Resumo

SIUCE M, Juan et al. Development of the recombinant fimbrial protein F17 of Escherichia coli and the immune response in alpaca peripheral blood mononuclear cells (PBMC). Rev. investig. vet. Perú [online]. 2021, vol.32, n.2, e20006. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v32i2.20006.

Escherichia coli is associated with diarrhoea that can cause death in young alpacas. Patotypes of E. coli use the fimbriae to adhere to the host in the first stage of pathogenesis. Therefore, the present study aimed to develop and produce a recombinant protein of E. coli fimbrial adhesin F17 and to evaluate its immunogenicity in alpaca peripheral blood mononuclear cells (PBMC). BL21 was used as an expression vector, the recombinant protein was purified by immobilized affinity chromatography and the production of PBMC cytokines was evaluated 48 and 72 hours after the challenge with F17. The results indicate a significant increase in the production of Th1/Th2 cytokines, predominantly IFN-γ and IL-4. Therefore, the BL21-F17 mixture could be considered as a potential vaccine candidate for the prevention of diarrhoea in alpacas produced by E. coli.

Palavras-chave : vaccine; Escherichia coli; F17; EHEC; alpaca.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )