Abstract

BOCANEGRA A., Alessandra et al. Detection and expression of retinoic X receptors (RXR) in the jejunal mucosa of baby alpaca (Vicugna pacos). Rev. investig. vet. Perú [online]. 2021, vol.32, n.3, e20388. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v32i3.20388.

The present study determined the presence of retinoic X receptor isoforms (RXR) genes in the genome of adult alpacas and their expression in the jejunal mucosa of alpaca offspring. Blood leukocyte samples from five adult alpacas were used to detect the genes of the RXRbeta and RXRgamma isoforms by PCR using specific primers, and to determine the expression of the RXR gene isoforms, 35 jejunum samples from young alpacas were used (1-50 days old), to which the real-time RT-PCR test was performed. The results indicate that the alpacas have encoded the RXRbeta and RXRgamma isoforms in their genome and that they have different levels of expression in the jejunal mucosa of baby alpacas. High expression of RXRgamma and RXRbeta was detected. These results indicate that the RXRgamma and RXRbeta receptors are being expressed in the mucosa of the jejunum of baby alpacas.

Keywords : X retinoic receptors; RXR; PCR; real time RT PCR; jejunal mucosa; pups; alpaca.

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