Abstract

REYES-GONZALEZ1, Erika Abigail et al. Effect of Ley-Leygo and Triladyl extenders and two equilibration times on the freezing of boar semen. Rev. investig. vet. Perú [online]. 2022, vol.33, n.6, e22758.  Epub Dec 22, 2022. ISSN 1609-9117.  http://dx.doi.org/10.15381/rivep.v33i6.22758.

The aim of this study was to evaluate the effect of Ley-Leygo and Triladyl® extenders and two equilibrium times on individual motility (IM), plasma membrane integrity (PMI), mitochondrial activity (MA) and intact acrosome (IA) in frozen-thawed boar semen. A rich fraction of a total of 20 ejaculates from five boars of a commercial line was used. All ejaculates had a minimum of 80% motility and 85% normal spermatozoa. Each ejaculate was divided into four aliquots: Ley-Leygo and Triladyl® extenders and two equilibrium times (0 and 1 h). The semen samples were thawed at 37 °C for 20 s. The results of the IM and the PMI did not have significant effects for the factors evaluated (p>0.05). There was a significant difference for MA (p<0.05) between the Ley-Leygo (43.57%) and Triladyl® (51.7%) diluents. The IA was 46% for Ley-Leygo and 25% for Triladyl® (p<0.05). The percentage of IA with the equilibration time of 1 h was greater than that of 0 h (p<0.05). It is concluded that the Triladyl® diluent improved the MA and the equilibration time of 1 h improved the percentage of IA.

Keywords : cryopreservation; frozen semen; reproduction; ejaculate.

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