Abstract

CHIA W., Julio A. et al. Diversidad genética molecular de Mirabilis expansa mediante RAPD: Molecular genetic diversity of Mirabilis expansa using RAP. Ecol. apl. [online]. 2006, vol.5, n.1-2, pp.81-86. ISSN 1726-2216.

A collection of 37 accessions of Mirabilis expansa, "chago" or "mauka" from northern Perú, was analyzed by RAPD assays and the molecular genetic diversity determined. In order to know which primer was more informative, the PIC values were summed up and a RAPD primer index calculated, resulting in the primers OPA04, OPA09 y OPA13 with the highest indexes, suggesting their potential utility in further research in Mirabilis species. From 9 of the 19 RAPD primers selected, we obtained 60 polymorphic markers, which yielded a Simple Matching Coefficient Dendogram, which shows 31 groups at a similarity index of 1. It also indicates a 16.216% of possible duplicates in the collection and with a similarity index of 0,85 we observed 8 clusters, with a significant difference compared to the morphological characterization data. An Analysis of Molecular Variance was performed, separating 2 variation components: among regions and among accessions/within region. The values obtained were 21,69% and 78,3% respectively, being both relatively high and showing considerable contribution of genetic variation from samples of diverse collection places.

Keywords : RAPD; Mirabilis expansa; Tuber roots; genetic diversity; AMOVA.

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