Abstract

SANCHEZ-TORRES, Heidi; JUSCAMAITA-MORALES, Juan; VARGAS-CARDENAS, Jessie  and  OLIVEROS-RAMOS, Ricardo. Production of the microalgae Nannochloropsis oculata (Droop) Hibberd on media enriched with biological fish ensilage. Ecol. apl. [online]. 2008, vol.7, n.1-2, pp.149-158. ISSN 1726-2216.

Fishing residues are produced in large volumes in Peru, generating pollution problems, but conversely can be a favourable substrate for biotechnological applications. Biological fish ensilage can replace massive culture media based on agricultural fertilizers for microalgae production. Nannochloropsis oculata was grown in batch culture during 12 days until stationary phase, under constant temperature and an illumination of 24 h.d-1. Seven different culture media (T1, Guillard F/2, T2, Yashima and T3-T7 d four culture media based on biological fish ensilage and T7 fish floor) were compared. Media based on biological fish ensilage were: two with different kinds of ensilages (hidrolized and non-hidrolized) and two with Yashima medium plus non-hidrolized ensilage at two different concentrations. The best experimental treatment was the medium based on non-hidrolized biological fish ensilage (T6), but non significant differences in maximum productivity were found compared with culture on Yashima medium (1.65 g.L-1.d-1). Monthly production using this medium was estimated in 41.5 g.month-1 .L-1. N. oculata biomass nutritional quality as live food for Brachionus plicatilis rotifer was evaluated and it was found that nonhidrolized ensilage treatment T6 had a greater fecundity index in comparison to Yashima treatment T2, which is an indicator of good nutritional quality. Biological fish ensilage medium showed a high production, low cost and good nutritional quality for the microalgae production.

Keywords : Aquaculture; fishing residue; organic medium; microalgae; Nannochloropsis; Brachionus.

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