Abstract

VALENCIA, Claudia  and  ZUNIGA, Doris. Analysis of natural mycorrhization in cotton crops (Gossypium barbadense L.) inoculated with Bacillus megaterium and/or Bradyrhizobium yuanmingense. Ecol. apl. [online]. 2015, vol.14, n.1, pp.65-69. ISSN 1726-2216.

For the process of protocol optimization, different staining process modifications proposed by Phillips & Hayman, which allow identification of intra-radicals structures derived from the plant-fungus interactions, were tested. For the said purpose, the roots of cotton plants (Gossypium barbadense L.) were extracted. These plants were previously inoculated with Bacillus megaterium (B), Bradyrhizobium yuanmingense (Br) separately and as well as in combination (I). In addition to this, a positive control with potassium nitrate (N+) and without it (N-) were also included. After the optimization of the staining procedure a count of the segments procedure was carried out to estimate the prevalence of infection by Arbuscular mycorrhizal (AM) in relation to the kind of fertilizers utilized. We found that six of the nine staining modifications allow the identification of the presence of mycorrhiza in roots of cotton plants, however, the variation D provides a better visualization of the morphological characteristics of AM. It was also noticed that the % LRC was low i.e. 28.77% in N+, while the mycorrhizal colonization was most favored when plants were inoculated with B, reaching an average of 70.98% LRC. Optimizations of standard protocols help reduce the cost and the exposure by the carcinogenic reagents. In conclusion, finding a relationship between AM and plant growth promoting rhizobacteria (PGPR) will help us to identify new ways of inoculation to increase production.

Keywords : B. megaterium; B. yuanmingense; PGPR; AM; G. barbadense L.

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