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Ecología Aplicada

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HUANUCO, Lucía; GUTIERREZ, Raymundo  and  MULLER, Giovanna. Standardization of a protocol for abscisic acid quantification through the competitive Elisa Tecnique. Ecol. apl. [online]. 2017, vol.16, n.1, pp.9-13. ISSN 1726-2216.

The most highlighted physiological response of plants to water stress by drought is the synthesis of the plant hormone Abscisic Acid (ABA). A solution to environmental changes is to achieve a better efficient use of water. For this reason, predicting the magnitude of ABA signaling may have considerable importance in agroecology; in addition, will allow the optimization of irrigation techniques in arid zones that need crops that limit the use of this natural resource. The most specific and reliable methodology for quantification of the enzyme is the indirect competitive type Enzyme- Linked Immunosorbent Assay (ELISA). For this reason the present study used this technique in order to establish a reproducible and accessible protocol that determines concentrations of ABA. The methodology included the evaluation of different antibodies, antigen and media concentrations. Results show that optimal conditions that allowed the highest sensitivity and reproducibility are: converage ABA-BSA conjugate 300 ng / ml, MAC 252 (monoclonal antibody specific for ABA) to 1:80 000, antirat IgG 1: 2000 and PNPP substrate in 1 mg / ml. Linear equations obtained gave R2 values in a range of 0.95-0.99 as well as significant differences between the positive and negative controls.

Keywords : water stress; drought; abscisic acid; Enzyme-Linked Immunosorbent Assay; MAC 252; agroecology.

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