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Ecología Aplicada
Print version ISSN 1726-2216
Abstract
SANTOS-MENDOZA, Ricardo et al. Production of riboflavin by lactic acid bacteria isolated from the manufacturing of "Tunta". Ecol. apl. [online]. 2018, vol.17, n.2, pp.185-189. ISSN 1726-2216. http://dx.doi.org/10.21704/rea.v17i2.1238.
In the present study, we selected and identified riboflavin-producing lactic acid bacteria (LAB), isolated ´during the process of manufacturing Tunta, one Peruvian native potato with traditional process of dehydration and freezing for comsumption and conservation. From 109 LAB isolates obtained, 32 were capable to produce riboflavin and only four showed a high level of the vitamin between 334 ng/ml and 426 ng/ml in riboflavin-free medium. The molecular characterization of the four strains allowed their identification with a 95% of similarity as Leuconostoc mesenteroides. Other strains were identified on the basis of 16S rRNA sequencing, as Lactobacillus curvatus, L. mesenteroides subsp. dextranicum and Lactobacillus sakei. Further studies with these riboflavin- producing LAB strains will allow to give value to Tunta´s nutrients as native Peruvian food.
Keywords : lactic acid bacteria (LAB); ribosomal gen 16S rRNA; riboflavin; tunta; Jallamilla.