Abstract

HUASASQUICHE SARMIENTO, Lucero; MORENO DIAZ, Patricia  and  JIMENEZ DAVALOS, Jorge. characterization and evaluation of pgpr potential of the microflora associated with Tarwi (Lupinus mutabilis Sweet). Ecol. apl. [online]. 2020, vol.19, n.2, pp.65-76. ISSN 1726-2216.  http://dx.doi.org/10.21704/rea.v19i2.1557.

Lupinus mutabilis, or tarwi, is a crop with a great cultural and nutritional importance. It has advantageous agricultural characteristics probably related to its native microflora. The aim was to characterize microflora associated with Lupinus mutabilis roots, stems, nodules and rhizosphere. Tests were performed according to PGPR capacities: indol acetic acid (IAA) production, tricalcic phosphates solubilization, siderophores production, antagonism against Fusarium oxysporum, in vitro fixation of nitrogen and tarwi seedlings growth effect. Microorganisms were obtained from plants growing in Lima and Jauja fields; we isolated 143 fungal strains and 190 bacterial strains. The most antagonist fungal strain inhibited 74% of the pathogen growth which was identified as Aspergillus. The IAA production test was used as initial screening in bacterial isolates, resulting in 36 strains with high and medium production. Then, we obtained up to 4.96 efficiency in phosphate solubilization test; 5.00 efficiency in the production of siderophores and up to 49.3% of inhibition in the antagonism test against a tarwi pathogen. The highest nitrogen fixation has found in strain NR05 with a concentration of 6 mg·l-1 of ammonium ion. Most of the bacterial isolates identified belong to the Bacillaceae family. Finally, the seedlings inoculated with strain TB05 reached the best values in plant height with an increase of 10% compared to the control. These results suggest the high potential of use in biofertilizers development from these microorganisms.

Keywords : Lupinus mutabilis; plant-microorganism interaction; PGPR capacities.

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