Revista Peruana de Medicina Experimental y Salud Publica
ISSN 1726-4634 versión impresa
MINAYA, G, TORRES, Y, FARFAN, M et al. Evaluación de la Intradermorreacción con Antígenos de Leishmania (Viannia) peruviana y Leishmania (Viannia) braziliensis en Áreas endémicas de Leishmaniasis en el Perú. Rev. perú. med. exp. salud publica, 1999, vol.16, no.1-2, p.5-14. ISSN 1726-4634.
Extracts of Leishmania soluble antigens (leishmanina) were made from Leishmania (Viannia) peruviana and Leishmania (Viannia) braziliensis promastigotes.Antigens were evaluated for sensitivity, specificity and side effects. Assays were perfomed in transmission areas for "uta" (andean leishmaniasis), "espundia" (sylvatic leishmaniasis), and also in patients seen in Lima. Leishmania (Viannia) peruviana antigen, 25 µg/ml, was adequate to detect late skin hypersensitivity reaction among patients with active cutaneous and/or mucocutaneous lesions, with an average sensitivity of 95,9%. The same sensitivity, but with more intense (potency) reactions were observed with the homologous 30 µg/ml antigen. Antigens of Leishmania (Viannia) braziliensis, 25 and 30 µg/ml, showed similar sensitivity and potency to the equivalent doses of Leishmania (Viannia) peruviana. The reaction’s sensitivity and potency increase minimally with increased protein concentration. No side effects, such as vesiculation and ulceration on the site of the innoculation, were observed. Antigens showed high specificity. Specificity in the control group reached 100,0% whereas in the endemic control group, it was 95,7%.
Palabras llave: Leishmaniasis; Leishmania; antigen; Peru.
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