Abstract

YABAR, C et al. Diagnóstico temprano del Virus Dengue 1 usando RT-PCR y perspectivas para la caracterización molecular  de Cepas Autóctonas. Rev. perú. med. exp. salud publica [online]. 1999, vol.16, n.1-2, pp.31-34. ISSN 1726-4634.

A diagnosis system for the early detection of Dengue virus was carried out by amplification of the NS1 gene portion using RT-PCR. Results obtained from clinical samples corresponded to previous RT-PCR works aimed at the structural region of the virion. Dengue cDNA from one of the sera samples was then cloned and sequenced. Comparison by nucleotide sequence analysis with other reference strains determined that this viral strain was Dengue serotype 1.

Keywords : Dengue; diagnosis; reverse transcriptase polymerase chain reaction.

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