Revista Peruana de Medicina Experimental y Salud Publica
versión impresa ISSN 1726-4634
GARCIA M, María et al. Determinación de Anticuerpos IgM contra Virus dengue partir de sangre absorbida en papel filtro : Un método alternativo y sencillo. Rev. perú. med. exp. salud publica [online]. 2000, vol.17, n.1-4, pp. 21-25. ISSN 1726-4634.
Objective: This study proposes the use of filter paper, in whole blood samples to test for Dengue, as an alternate method, due to the fact that it is very simple and does not require of much detail for shipping samples to the laboratory. Materials and Methods: Using vacutainers and filter papers, whole blood sample obtained simultaneously from 100 patients diagnosed as classic dengue. All samples were tested using IgM capture ELISA. Findings: Out of 100 samples, 25 were positive and 75 were negative in sera, in filter paper 24 positive and 74 negative, with a Kappa concordance index of 0,97 and a sensitivity and specificity of 96,0% and 98,0% respectively: the positive predictive value was 96.0% and the negative predictive value was 98,0%. Conclusions: A very good sensitivity, specificity and concordance in the determination of IgM antibodies against Dengue is evidenced using filter paper when obtaining blood samples.
Palabras llave : Dengue/diagnosis; Paper filters; Enzyme-Iinked immunosorbent assay.