Abstract

YABAR V, Carlos  and  MONTOYA P, Ysabel. Síntesis in vitro de la proteína de la envoltura del virus peruano de la fiebre amarilla. Rev. perú. med. exp. salud publica [online]. 2001, vol.18, n.1-2, pp.9-13. ISSN 1726-4634.

Objective: To synthesise envelope recombinant protein (Er) from Peruvian yellow fever virus (FA) by using molecular approaches. Materials and Methods: The gene that codes this protein was analysed by RT-PCR, then cloned into a plasmid vector and sequenced by using DNA sequencing. Later, the DNA insert was subdoned into an expression vector to be translated to protein. Results: The protein was purified through an affinity column under denaturing conditions and visualised by SDS-PAGE electrophoresis. Results: The recombinant protein was 66 kDa molecular weight and it was obtained about 10 mg/ml from 1x108 cell (OD=0.5) after three hours of induction. Conclusion: Amino acid sequence analysis showed that Er might be a good candidate to be tested using sera and then to be used as a specific yellow fever diagnosis tool.

Keywords : Yellow fever; Protein/isolation & purification; Reverse transcriptase polymerase chain reaction.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )