Abstract

PONCE G, Carlos  and  SOLORZANO E, Nelson. Evaluación de la transmisión vertical de Bartonella bacilliformis en Lutzomyia verrucarum (Diptera: Psychodidae). Rev. perú. med. exp. salud publica [online]. 2002, vol.19, n.2, pp.93-96. ISSN 1726-4634.

Objectives: To determine vertical transmission of Bartonella bacilliformis in Lutzomyia verrucarum. Materials And methods: In this experimental study, we performed individual and massive breeding (Tº: 22 ± 2º C, relative humidity: 80 ± 5%) of Lutzomyia verrucarum at the entomology laboratory in Caraz Hospital (Ancash - Peru). In order to infect female mosquitoes, we fed them with blood from patients with positive-smear acute bartonellosis. Female mosquitoes were assessed after they laid their eggs, using a PCR test for Bartonella baciliformis. Results: Thirteen of 18 (72.2%) females fed with infected blood with 3% bacteremia laid their eggs, and none of them was PCR positive. Twelve of 54 (22.2%) females fed with infected blood, with 80% bacteremia laid their eggs, and only one (8.3%) was PCR positive. No adult offspring of this female was PCR positive. Conclusions: The low rate of Bartonella baciliformis infection in female mosquitoes laying their eggs did not show the existence of vertical transmission of Bartonella bacilliformis in Lutzomyia verrucarum.

Keywords : Bartonella; Psychodidae; Bartonella infections/transmisión; Disease transmission; vertical; Polymerase chain reaction.

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