SciELO - Scientific Electronic Library Online

vol.20 issue1Estudio basal de prevalencia de sífilis y VIH y comportamientos asociados en población privada de libertad, Perú 1999 author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Revista Peruana de Medicina Experimental y Salud Publica

Print version ISSN 1726-4634


PADILLA R, Carlos  and  VENTURA E, Gladys. Diseño y estandarización de una prueba de PCR para el diagnóstico de la  Bartonelosis causada por Bartonella bacilliformis. Rev. perú. med. exp. salud publica [online]. 2003, vol.20, n.1, pp.5-8. ISSN 1726-4634.

Objective: To design a PCR test for the diagnosis of Bartonellosis produced by Bartonella bacilliformis. Material and methods: ialB locus was used to design ialBF and ialBR primers, and PCR conditions were standardized using genomic DNA from a referral strain. Furthermore, the test was preliminary assessed in 12 clinical isolates from three Peruvian endemic areas and 10 whole blood samples of bartonelosis confirmed cases. Results: This test detected DNA from B. bacilliformis strains from three Peruvian endemic places of bartonellosis: Ancash, Cuzco and Lima. Genomic DNA of B. hensenlae, B. vinsonii, and other pathogens were not detected. In addition, this test was positive for ten whole blood samples of patients with confirmed bartonellosis and it was negative for five whole blood samples of patients with P. falciparum malaria. Conclusions: This PCR test could be useful for diagnosing bartonellosis caused by B.bacilliformis.

Keywords : Bartonella infections; Polymerase chain reaction.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License