Abstract

PADILLA R, Carlos  and  VENTURA E, Gladis. Genotipificación de aislamientos de Bartonella bacilliformis por amplificación de elementos repetitivos mediante el uso de REP-PCR y ERIC-PCR. Rev. perú. med. exp. salud publica [online]. 2003, vol.20, n.3, pp.128-131. ISSN 1726-4634.

Objetive: To genotype Bartonella bacilliformis isolates by repeated elements amplification using ERIC-PCR and REP-PCR, and to determine if there is any genetic variability among isolates from several endemic areas. Materials y Methods: 17 B. bacilliformis isolates from Lima, Cusco and Ancash were assessed using ERIC-PCR and REP-PCR. Fingerprintings of the samples were obtained, and they were analyzed using GelCompar 4,0 software. Results: 10 genotypes were identified. Genotypes D, E and H were detected in Cusco; while genotypes B, C, G, J and I were detected in Lima; and genotype F was found in Ancash. Conclusions: Our results indicate that REP-PCR and ERIC-PCR are useful methods for genotyping B. bacilliformis isolates. Genetic polymorphism should be taken into account in epidemiological and clinical studies, as well as for the development of new vaccines and diagnostic methods.

Keywords : Bartonella bacilliformis; Polymerase Chain Reaction; Bartonella infections; Genotype.

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