Revista Peruana de Medicina Experimental y Salud Publica
versión impresa ISSN 1726-4634
MAMANI Z, Enrique et al. Molecular typing of dengue 3 virus during the classic dengue outbreak in Lima-Peru, 2005. Rev. perú. med. exp. salud publica [online]. 2005, vol.22, n.3, pp. 161-164. ISSN 1726-4634.
Objectives: To identify the causative agent of the outbreak that occurred in Comas, Lima in April 2005 using reverse transcription-polymerase chain reaction (RT-PCR) and specific restriction site-PCR (RSS-PCR). Materials and methods: 20 serum samples collected during the dengue outbreak were assessed using RT-PCR for identifying serotypes, this technique was performed in a single step. Later, the RSS-PCR method was used to identify the circulating serotypes, and the results were corroborated by viral isolation and sequencing. Results: RT-PCR of viral RNA taken from the samples showed a 290-pb amplified product corresponding to dengue virus serotype 3 (DEN 3). RSS-PCR product analysis of RNA from DEN 3 isolates corresponded to pattern C, included in genotype III. Dengue 3 virus isolates in C6/36 cell lines identified using indirect immunofluorescence and gene sequencing confirmed the results obtained using the aforementioned tests. Conclusion: During the classic dengue fever outbreak in Lima, DEN 3 genotype III circulated.
Palabras llave : Outbreak of classic dengue; dengue 3 virus; molecular typing; RT-PCR; RSS-PCR; Peru (source: DeCS BIREME).